Catalogue | DB_006

Lacey's PluriPlus

Media supplement optimised to help pluripotent cells - particularly porcine induced pluripotent stem cells (piPSCs) - maintain their pluripotent state during expansion. Suitable for use in adherent and suspension-based expansion processes. Can be added to almost any media formulation.

Lacey's PluriPlus
Reliable

Reliable

No more loss of pluripotency or uncontrolled differentiations when you're expanding your cells

Versitile

Versitile

Versitile

Flexible

Flexible

You can add Lacey's PluriPlus to any commercially available medias, or your own proprietary formulations.

Lacey's PluriPlus
Dragon Biotechnologies
00:00
/
00:00


Stem cells - particularly induced pluripotent stem cells (iPSCs)- have great potential as a starting source for cell manufacturing processes being functionally immortalised and able to differentiate into almost any cell type. However, a key challenge is ensuring  iPSCs maintain their pluripotency or 'stemness' to avoid uncontrolled differentiation or cell death.

When working with porcine iPSCs. We found maintaining pluripotency to be an ongoing challenge. So we created Lacey's PluriPlus. A simple-to-add supplement for your culture media, Lacey's PluriPlushelps porcine iPSCs maintain their pluripotency in adherent and suspension culture conditions in the lab, and in manufacture.

Product information

Performance in Anchorage Dependent Culture Conditions

Lacey's PluriPlus was first tested in an anchorage dependent porcine iPSC expansion protocols.

Lacey's PluriPlus was added to two commercially available medias for supporting stem cell expansion. With Lacey's PluriPlus, porcine iPSCs in both medias showed more homogenous and normalised expression of markers for pluripotency.

Both medias with Lacey's PluriPlius exhibited simmilar growth curves over a multi-day expansion protocol.

Performance in Suspension Adapted Culture Conditions

Lacey's PluriPlus was also tested in a protocol adapting porcine iPSCs to suspension culture conditions.

Porcine iPSCs were adapted to suspension culture conditions by the formation of aggregates or embryoid bodies, then expanded over a four day protocol. iPSCs cultured in commercially available medias enriched with Lacey's PluriPlus showed consistent expression of OCT4 and SOX2, indicating the iPSCs were in a pluripotent state.