Catalogue | DB_004

Bovine Immortalised Adipose Derived Stem Cells

This biADSC line is an immortalised adipocyte-derived stem cell line. Our first bovine-derived cell line is suitable for both basic R&D and production.

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Bovine Immortalised Adipose Derived Stem Cells
Consistent

Consistent

Homogenous adipogenic cell population creates a stable model for foundational and applied research .

Functional

Functional

biADSCs display key differentiation profiles for use in discovery and applied research

Easy to Use

Easy to Use

Simple to follow expansion and differentiation protocols

Bovine Immortalised Adipose Derived Stem Cells
Dragon Biotechnologies
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Cell Characterization

biADSC growth data. Top) representative image of biADSCs and positive expression for the SV40T antigen. Bottom) Representative cell growth curve over 5 days of culture

Following enzymatic digestion of fresh porcine abdominal adipose tissue, cells were cultured on treated cell culture plastic for 2 passages to isolate and expand the ADSC population. ADSCs were immortalised by incubation with Lentivirus containing the SV40 large T antigen for 24 hours. Immortalised cells were selected by treatment of cultures with neomycin for 7 days. Lentiviral vectors are integrative so the expression of the SV40 large T antigen is constitutive. The SV40 large T antigen suppresses the expression of the cell cycle regulator P53 locking cells in the cell cycle. biADSCs test positive for the expression of the SV40 large T antigen and negative for residual Lentivirus. Following immortalisation biADSCs retain their capacity for adipogenic differentiation. biADSCs are cultured in high glucose DMEM containing 10% FBS.

Differentiation Characterization

Adipogenic differentiation of biADSCs Top) Oil Red O staining of differentiated biADSC compared to undifferentiated biADSCs. Bottom) Expression of the adipogenic marker genes PPARG and ADIPOQ in differentiated biADSCs compared to undifferentiated.

Compatible Processes

Cell Growth Formats
Adherent
Cell Culture Vessels
Well Plates
Flasks

Product information

24-30 hours
Avg. Population Doubling Time
Adipogenic Differentiation
0 days
10-20 days
Immortalisation Method
Lentiviral SV40 Large T Antigen
Species (Latin)
Bos taurus taurus
Founder Animal
Male Hereford
Working Cell Bank Passage Number
P5-P10
Maximum Passage Number Achieved
P18
Quality Control
Sterility and mycoplasma testing
Format

Cryopreserved cells, one vial containing 1x106 cells

User Storage
-150°C or LN2
Shipping Method
Dry ice for immediate ultra-cold storage

Industry use-cases

Cultivated Meat
Cultivated Meat
Beef up your fundamental discovery, enabling technology research and commercial cultivated meat product development with a suite of tailor-made solutions.

Key Applications

Species Specific Growth Factor Testing
When developing a growth factor, media formulation, or any tool or technology designed to be used in the culturing and production of animal cell lines, it is imperative to use a species-specific cell line model in your lab to generate physiologically relevant data on how your innovation will interact with the cell systems that will be used in production.

Related resources

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