Porcine immortalised adipose-derived stem cells
This piADSC line is an immortalised adipocyte-derived stem cell line. These cells have a rapid (<12 hours) average population doubling time, can be adapted to adherent and suspension growth conditions in serum and serum free media formulations. They are easy to handle and are suitable for both enabling and scalable cultivated fat product development.
Product Features
Quick
Rapid population doubling times.
Scalable
Adaptable to a range of scalable bioprocess conditions and medias to fit your R&D, regulatory and commercial needs.
Easy to Use
Simple to follow expansion and differentiation protocols. These cells grow like mad!
Following enzymatic digestion of fresh porcine abdominal adipose tissue, cells were cultured on treated cell culture plastic for 2 passages to isolate and expand the ADSC population. ADSCs were immortalised by incubation with Lentivirus containing the SV40 large T antigen for 24 hours. Immortalised cells were selected by treatment of cultures with neomycin for 7 days. Lentiviral vectors are integrative so the expression of the SV40 large T antigen is constitutive. The SV40 large T antigen suppresses the expression of the cell cycle regulator P53 locking cells in the cell cycle. piADSCs test positive for the expression of the SV40 large T antigen and negative for residual Lentivirus. Following immortalisation piADSCs retain their capacity for adipogenic differentiation.
Cell characterization
Suspension adaptation
Differentiation characterization
Compatible Processes
Product information
Cryopreserved cells, one vial containing appx. 1 x 106 cells
Species specific growth factor testing: QKine x Dragon
Species specific growth factor testing: QKine x Dragon