Catalogue | DB_003

Porcine Immortalised Myosatellite Cells

This piMyoSat line is an immortalised cell line with a rapid population doubling and differentiation protocol time, piMyoSat’s will strengthen and accelerate your discovery work, and are suitable for use in cultivated muscle production.

Consistent

Homogenous myogenic cell population creates a stable model for foundational and applied research

Easy to Use

Simple to follow expansion and differentiation protocols.

Quick

Rapidly form striated myocytes in days to accelerate your R&D

Porcine Immortalised Myosatellite Cells

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Cell Characterization

piMyoSat growth data. Top) representative image of piMyoSats and positive expression for the SV40T antigen. Bottom) Representative cell growth curve over 5 days of culture.

Following enzymatic digestion of fresh porcine abdominal skeletal muscle tissue, cells were cultured on treated cell culture plastic to deplete cell suspensions of fibroblasts after which they were transferred onto type I collagen coated surfaces to select for myosatellite cells. Cultures were maintained for 2 passages to expand the myosatellite population. Myosatellite cells were immortalised by incubation with Lentivirus containing the SV40 large T antigen for 24 hours. Immortalised cells were selected by treatment of cultures with neomycin for 7 days. Lentiviral vectors are integrative so the expression of the SV40 large T antigen is constitutive. The SV40 large T antigen suppresses the expression of the cell cycle regulator P53 locking cells in the cell cycle. piMyoSats test positive for the expression of the SV40 large T antigen and negative for residual Lentivirus. Following immortalisation piMyoSats retain their capacity for myogenic differentiation. piMyoSats are cultured on type I collagen coated surfaces in high glucose DMEM containing 10% FBS.