Catalogue | DB_001

Porcine Induced Pluripotent Stem Cells

This porcine iPSC line was reprogrammed from intramuscular fibroblasts and the cells available are banked at P20+. They have been tested for sterility, mycoplasma, expression of pluripotency markers and differentiation to ectoderm, mesoderm and endoderm by embryoid body differentiation. Upon revival, the cells can be differentiated using our validated protocols to adipocytes and multinucleated muscle cells. piPSCs provide a source of consistent, reliable cells for both R&D and production. Ask us about our enhanced data packs.

Porcine Induced Pluripotent Stem Cells
GMO-free

GMO-free

Streamlined regulatory compliance for products in jurisdictions where genetically modified organisms.

Pluripotent

Pluripotent

Able to differentiate into almost any cell type, including adipocytes (fat) and myocytes and myofibers

Streamlined

Streamlined

Single starting cell for the creation of cell line models and product development

Porcine Induced Pluripotent Stem Cells
Dragon Biotechnologies
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Cell Characterization

Pluripotency marker expression for piPSCs. Top) immunofluorescent staining for OCT4 and NANOG. Bottom) flow cytometry for OCT4, SOX2 and SSEA4

This piPSC line was derived by the reprogramming of intramuscular fibroblasts using a non-integrative method to deliver human isoform c-Myc, Klf4, Oct4 and Sox2. piPSCs are adapted to feeder free culture on commercially available basement membrane extract. In order to maintain pluripotency piPSCs are cultured in a commercially available maintenance medium with additional growth factor/small molecule supplementation.

Differentiation Characterization

Differentiation of piPSCs. Top) Embryoid bodies formed from piPSCs positive for the expression of the germ layer markers PAX6 (ectoderm), T (mesoderm) and SOX17 (endoderm). Middle) piPSCs differentiated to adipocytes were positive for Oil Red O staining and the marker genes PPARG and CEBPB with diminished expression of OCT4. Bottom) piPSCs differentiated to skeletal muscle were positive for multinucleated fibres and the marker genes MyoD1 and MYH2 with diminished expression of OCT4.

Compatible Processes

Cell Growth Formats
Adherent
Cell Culture Vessels
Well Plates
Flasks
Adherent Scale Up

Product information

24 hours
Avg. Population Doubling Time
Adipogenic differentiation
0 days
28 days
Immortalisation Method
Induced pluripotency via non integrative reprogramming method.
Species (Latin)
Sus scrofa domesticus
Founder Animal
Male Duroc white
Working Cell Bank Passage Number
P20+
Maximum Passage Number Achieved
P50
Quality Control
Sterility and mycoplasma testing
Format

Cryopreserved cells, one vial containing appx. 2 x 106 cells

User Storage
-150°C or LN2
Shipping Method
Dry ice for immediate ultra-cold storage