Porcine Spontaneously Immortalised Adipocyte Derived Stem Cells
Spontaneously immortalised stem cells derived from porcine primary adipose tissue. These cells were treated using a non-integrative, non-GMO method to induce spontaneous immortalisation. The cells have a rapid (<17 hours) average population doubling time, can be adapted to adherent and suspension growth conditions in serum and serum free media formulations. They are easy to handle, and are suitable for enabling technology discovery and scalable cultivated fat product development.
Non-GMO
Spontaneously Immortalised cell line using non integrative, non GMO techniques.
Immortalised
Functional immortalization ensures robust phenotype and performance over tens to hundreds of population doublings.
Suspension Adapted
Adaptable to micro-carrier, aggregate and single cell suspension culture conditions.
Cell Characterization
Following enzymatic digestion of fresh porcine abdominal adipose tissue, cells were cultured on treated cell culture plastic for 2 passages to isolate and expand the ADSC population. ADSCs were spontaneously immortalised using two methods. Following immortalisation psiADSCs retain their capacity for adipogenic differentiation. psiADSCs are cultured in high glucose DMEM containing 10% FBS, but can be adapted to serum free media formulations.
Differentiation Characterization
Compatible Processes
Product information
Cryopreserved cells, one vial containing appx. 1 x 106 cells
Species Specific Growth Factor Testing: QKine x Dragon
Species Specific Growth Factor Testing: QKine x Dragon