Catalogue | DB_005

Porcine spontaneously immortalised adipocyte derived stem cells

Spontaneously immortalised stem cells derived from porcine primary adipose tissue. These cells were treated using a non-integrative, non-GMO method to induce spontaneous immortalisation. The cells have a rapid (<17 hours) average population doubling time, can be adapted to adherent and suspension growth conditions in serum and serum free media formulations. They are easy to handle, and are suitable for enabling technology discovery and scalable cultivated fat product development.

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Porcine spontaneously immortalised adipocyte derived stem cells

Product Features

Non-GMO

Non-GMO

Spontaneously Immortalised cell line using non integrative, non GMO techniques.

Immortalised

Immortalised

Functional immortalization ensures robust phenotype and performance over tens to hundreds of population doublings.

Suspension Adapted

Suspension Adapted

Adaptable to micro-carrier, aggregate and single cell suspension culture conditions.

Porcine spontaneously immortalised adipocyte derived stem cells
Dragon Biotechnologies
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Following enzymatic digestion of fresh porcine abdominal adipose tissue, cells were cultured on treated cell culture plastic for 2 passages to isolate and expand the ADSC population. ADSCs were spontaneously immortalised using two methods. Following immortalisation psiADSCs retain their capacity for adipogenic differentiation. Suspension adapted psiADSCs have been further adapted to serum free media conditions.
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Cell characterization

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qPCR to asses fold change of markers indicating functional immortalisation of the psiADSCs. MK67 is a marker fo proliferation. PRIM2 is a marker of DNA replication. Caspase 3 is a marker of cell apoptosis. SQS is a checkpoint inhibitor. SMC3 is a maker of structural maintenance and cell cycle. And MCM2 is a marker of gene replication and cell cycle.

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Suspension adaptation

Adaption of psiADSCs to suspension culture conditions. Left) psiADSCs grown in 150mL bottles in suspension adapted conditions. Single cells can be observed, with some small aggregates formed. Further bioprocess optimization could increase the homogeneity of singled cells and desired process parameters. Right) psiADSCs expanding on gelatin coated microcarriers.

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Differentiation characterization

Differentiation of psiADSCs into adipocytes. Left) brightfield image of differentiated psiADSCs. Accumulation of lipid molecules can be seen in differentiated cells. Right) Oil-O-Red staining of differentiated psiADSCs. Cells were fixed for 15 minutes in 4% PFA solution and stained for 30 minutes in Oil-O-Red solution at 10 days after differentiation protocol was initiated. Red staining indicates lipids in all cells, and clusters of lipids can be observed in most cells.

Compatible Processes

Cell Growth Formats
Adherent
Single Cell Suspension
Suspension Adapted
Cell Culture Vessels
Adherent Scale Up
Flasks
Scale Down Suspension Systems
Well Plates

Product information

18-24 hours
Avg. Population Doubling Time
Adipogenic Differentiation Protocol
0 days
8-10 days days
Immortalisation Method
Spontaneous Immortalisation
Species (Latin)
Sus scrofa domesticus
Founder Animal
Male Duroc white
Working Cell Bank Passage Number
Banks at P30+
Maximum Passage Number Achieved
P50+
Quality Control
Sterility and mycoplasma testing
Format

Cryopreserved cells, one vial containing appx. 1 x 106 cells

User Storage
-150°C or LN2
Shipping Method
Dry ice for immediate ultra-cold storage

Industry use-cases

Drug development
Drug development
Do you need an early indication of your drug or therapeutic candidate's toxicology profile in large animals but aren't ready to commit months of your precious time and six-seven figure sums to run a pre-clinical study? Read on.
Cultivated meat
Cultivated meat
Beef up your fundamental discovery, enabling technology R&D and cultivated meat product development with solutions tailor-made for you.

Key Applications

Species specific growth factor testing
When developing a growth factor, media formulation, or any tool or technology designed to be used in the culturing and production of animal cell lines, it is imperative to use a species-specific cell line model in your lab to generate physiologically relevant data on how your innovation will interact with the cell systems that will be used in production.

Relevant Media Products

More media products are coming soon!

Related resources

Check out some of the resources we have available related to Porcine spontaneously immortalised adipocyte derived stem cells.
Dragon Biotechnologies
Species specific growth factor testing: QKine x Dragon
Posted Mar 18, 2024
Resource
App Notes

Species specific growth factor testing: QKine x Dragon

Showcasing the efficacy of using QKine's growth factors (FGF2, TGFβ1 and Activin A) to maintain piPSC pluripotency during culture
Read more
Species specific growth factor testing: QKine x Dragon
Dragon Biotechnologies
Posted Mar 18, 2024
Resource
App Notes

Species specific growth factor testing: QKine x Dragon

Showcasing the efficacy of using QKine's growth factors (FGF2, TGFβ1 and Activin A) to maintain piPSC pluripotency during culture
Download File
Species specific growth factor testing: QKine x Dragon
Dragon Biotechnologies
Posted Mar 18, 2024
Resource
App Notes

Species specific growth factor testing: QKine x Dragon

Showcasing the efficacy of using QKine's growth factors (FGF2, TGFβ1 and Activin A) to maintain piPSC pluripotency during culture
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