Suspension-Adapted Porcine Immortalised Myosatellite Cells
DB_003s suspension-adapted porcine immortalised myosatellite cells (Suspension-adapted piMyoSats) are TERT-immortalised myosatellite cells adapted for growth as aggregates in suspension culture. These cells retain robust proliferative capacity and efficiently undergo myogenic differentiation in suspension conditions. Suspension-adapted piMyoSats exhibit rapid growth, with an average population doubling time of less than 24 hours and are optimised for scalable cultivated muscle production, cellular agriculture research, and enabling technology development.
Product Features
Robust Long-Term Proliferation
Suspension-adapted immortalised porcine muscle stem cells with rapid growth kinetics and average doubling times of less than 24 hours.
Suspension-Adapted
Optimised for aggregate-based suspension culture systems and scalable bioreactor expansion workflows.
Stable Myogenic Phenotype
Maintain expression of key skeletal muscle stem cell markers during suspension culture and efficiently differentiates into multinucleated Myosin Heavy Chain (MHC)-positive myotubes.
Cell Source and Immortalisation
Porcine myosatellite cells were isolated from abdominal skeletal muscle by enzymatic digestion and purified by flow cytometry for CD29⁺/CD56⁺/CD31⁻/CD45⁻ cells. Purified cells were expanded on laminin-521-coated flasks and passaged at least twice prior to immortalisation using TERT (telomerase reverse transcriptase). Following immortalisation, piMyoSats were adapted to suspension culture over 10 sequential passages until cell viability consistently exceeded 90% and robust proliferative capacity in suspension culture was established.
Cell Characterisation
Suspension-adapted piMyoSats grow as aggregates in suspension culture (Figure 1A) and exhibit rapid proliferation over a 4-day culture period (Figure 1B). Cells retain skeletal muscle stem cell identitythrough continued expression of the canonical myogenic marker PAX7 (Figure 1C).
Myogenic Differentiation Characterisation
Suspension-adapted piMyoSats retain robust myogenic lineagepotential following suspension adaptation. Upon differentiation, cellsefficiently form multinucleated, Myosin Heavy Chain (MHC)-positive myotubes,confirming successful skeletal muscle differentiation (Figure 2).
piMyoSats were differentiated for 7 days and subsequently stainedfor Myosin Heavy Chain (MHC) and DAPI nuclear counterstain. Robust MHC-positivestaining confirms successful myogenic differentiation and multinucleatedmyotube formation.
Compatible Processes
Product information
Cryopreserved cells, one vial containing appx. 2 x 106 cells
