Suspension-Adapted Porcine Spontaneously Immortalised Adipose-Derived Stem Cells
Suspension-adapted porcine spontaneously immortalised adipose-derived stem cells (DB_005s) are engineered for robust proliferation and adipogenic differentiation in suspension culture. Derived from primary porcine adipose tissue, these cells were spontaneously immortalised using a proprietary non-integrative, non-GMO method and subsequently adapted to single-cell suspension culture. DB_005s cells exhibit rapid growth, with an average population doubling time of approximately 24 hours and can be maintained under single-cell suspension culture conditions. The cell line is highly suitable for scalable cultivated fat production, bioprocess optimisation, and cellular agriculture technology development.
Product Features
Suspension Adapted
Adapted for robust single-cell suspension culture conditions while maintaining high viability and proliferative capacity.
Efficient Adipogenic Differentiation
Retains adipogenic lineage potential and differentiates into lipid-accumulating adipocytes under suspension differentiation conditions.
Non-GMO Immortalised
Spontaneously immortalised fast-growing suspension cell line
Cell Source and Immortalisation
Primary porcine adipose-derived stem cells (ADSCs)were isolated from subcutaneous abdominal adipose tissue using enzymatic digestion methods. Cells were expanded on tissue culture-treated plastic for a minimum of two passages prior to immortalisation. Primary porcine ADSCs were subsequently subjected to a proprietary spontaneous immortalisation process designed to enable long-term proliferative capacity while maintaining adipogenic functionality.
Cell Characterisation
Spontaneously immortalised porcine adipose-derived stem cells (psiADSCs) were adapted to suspension culture over 10 sequential passages until robust proliferative growth in suspension was achieved. Suspension-adapted psiADSCs grow predominantly as single cells in suspension culture (Figure 1A) and demonstrate rapid proliferation over a 4-day growth period (Figure 1B).
(A) Representative brightfield microscopy image showing suspension-adapted psiADSCs growing as a single-cell suspension. (B) Growth curve demonstrating robust proliferation of suspension-adapted psiADSCs over a 4-day period.
Adipogenic Differentiation in Suspension
Importantly, suspension-adapted psiADSCs retain strong adipogenic differentiation potential.Following a 14-day adipogenic differentiation protocol in suspension culture, cells develop intracellular lipid accumulation and differentiate into Oil RedO-positive adipocytes (Figure 2).
Representative microscopy images showing adipogenic differentiation of suspension-adapted psiADSCs following Oil Red O staining. Intracellular lipid accumulation is observed in differentiated cells compared with undifferentiated control cells.
Compatible Processes
Product information
Cryopreserved cells, one vial containing appx. 2 x 106 cells
